In-depth rheological characterization of genetically ...
In detail, 50 ml of cell cultures were grown to an OD 600 between 0.6 and 1.0, centrifuged (7500 g, 20 °C, 5 min) in a 50 ml conical tube, the pellet was washed in 20 ml 250 mM sucrose 3 times by resuspending and centrifugation (7500 g, 20 °C, 5 min) and finally resuspended in 1 ml of 250 mM sucrose. 100 μl aliquots were used right away for ...